cDNA Libraries and Expression Libraries

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Session Overview


This session will review how to make a recombinant cDNA library and how to use this library to find a specific gene. This session will outline the differences between a genomic and a cDNA library, and discuss how to use a cDNA library to clone a gene of interest.

Learning Objectives

  • To understand what a recombinant cDNA library is and how it is constructed.
  • To understand how to use a cDNA library to identify a gene of interest by the protein made.

Session Activities

Lecture Video

Watch the lecture video excerpt

Check Yourself



Session Activities

Help Session Video

Watch the short video of Robert Dorkin explaining transformation and protein expression.

Practice Problems

Further Study

Useful Links


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